Figure 1.

Experimental setup and transcriptional profile of myeloid cells during aging and CNV formation. (A) Scheme illustrating experimental setup. Myeloid cells were isolated from six-week-old or two-year-old Cx3cr1^Gfp/+ mice, either without laser treatment or three days following laser CNV induction. (B) Isolation of myeloid cells via fluorescence-activated cell sorting. Myeloid cells were defined as CD45^lowCD11b⁺Cx3cr1-GFP⁺Ly6C⁻Ly6G⁻ and isolated from the retinae of young and old mice without laser induction (young: ocher; old: light green) and three days after laser induction (young: blue; old: red). (C) Principal component analysis (PCA) illustrating the clustering of myeloid cells from the four different groups (young control: ocher; old control: light green; young CNV: blue; old CNV: red). (D) Heatmap visualizing differentially expressed genes between young and old control groups, as well as between young CNV and old CNV groups. The z-score represents a gene’s expression in relation to its mean expression by standard deviation units (red: upregulation; blue: downregulation).