Figure 2.

The cycle of scaffold fabrication and implantation. The anatomic contour of the medial meniscus was obtained from a live human donor, who had undergone total knee replacement, and a custom-made meniscal scaffold was fabricated for a total graft (A), with layer-by-layer deposition of silicone resin using 3D printing technology (B). The meniscal scaffold was bioactivated with primary or stem cells and growth factors, and cultured in a bioreactor, stimulating at least one aspect of the in vivo environment (C). The final step was the meniscal scaffold implantation (D).