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. 2021 Dec 3;13(12):2421. doi: 10.3390/v13122421

Figure 1.

Figure 1

Experimental setup of five bank vole experiments. Four bank voles from the island of Riems, Mecklenburg-Western Pomerania (FA07 and F208), and close to Gotha, Thuringia (KS13/914 and KS13/920), were initially identified to carry BvHVs by RT-qPCR. RT-qPCR-mediated typing and high-throughput sequencing revealed single infection with Hepacivirus F (FA07), Hepacivirus J (F208 and KS13/920) and a double infection with both BvHVs (KS13/914). Blood or visceral cavity lavage of these animals was used to inoculate bred bank voles intraperitoneally (i.p.) and intramuscularly (i.m.) in experiment 1. Experiment 2 was performed with mice inoculated with liver suspension with wt-FA07, wt-F208 and bank vole passaged FA07 (p1-FA07; a pool of liver suspensions of voles 1-1 to 1-4 from experiment 1). Further experiments were performed with i.p. inoculation of this p1-FA07 or of original wt-F208 strain. Numbers in the vole graph indicate the number of individuals per trial. All control groups were omitted from the graphs.