Table 2.
The list of induced proteins in response to alkaline stress in the Y. lipolytica yeast using mass spectrometry (MALDI-TOF MS and MS/MS) under alkaline conditions.
| Spot Number | Protein Name | NCBI or UniProt ID | S/M/C * | MM/pI (expt.) ** |
MM/pI (calc.) ** |
|---|---|---|---|---|---|
| 24 ‡ | YALI0B09339p, endopeptidase activator activity (281 a.o.) |
Q6CF86 | 79/7/43 | 31.0/4.6 | 31.1/4.5 |
| 25 | YALI0D00451p, Uncharacterized protein (584 a.o.) |
Q6CAS0 | 178/13/21 | 57.0/7.7 | 57.1/6.6 |
| 27 ‡ | YALI0B07007p, NAD(P)H dehydrogenase (quinone) activity (203 a.o.) |
Q6CFH1 | 81/12/87 | 22.0/4.8 | 20.2/5.30 |
| 27 ǂ | YALI0D08184p, SSA4 heat shock protein of HSP70 family, fragment | Q6C9V0 | 115/27/28 | 21/4.8 | 70.3/4.9 |
| 28 | YALI0F08327p (139 a.o.) |
Q6C2F6 | 111/7/51 | 22.0/4.6 | 14.7/4.6 |
| 29 ‡ | YALI0A10879p E3 ubiquitin ligase complex SCF subunit (162 a.o.) |
XP_499961 | 84/4/43 | 18.5/0/4.6 | 18.3/4.4 |
| 30 ‡ | YALI0E32736p, Bis(5′-adenosyl)-triphosphatase (169 a.o.) |
XP_002143095.1 | 163/13/69 | 17.7/5.1 | 18.7/5.2 |
| 31 ‡ | Calcium binding protein YALI0E03388p (147 a.o) |
Q6C765 | 162/15/79 | 17.0/4.7 | 16.8/4.8 |
| 34 | YALI0A10747p (Ribosome maturation protein family) (117 a.o.) |
Q6CHA5 | 193/10/78 | 13.0/5.5 | 13.1/5.2 |
| 35 ‡ | YALI0C03443p (small heat shock protein (HSP20) family) (149 a.o.) |
Q6CD64 | 135/13/79 | 17.5/6.2 | 17.3/5.6 |
| 36 | YALI0F08393p, Nascent polypeptide-associated complex subunit beta (162 a.o.) |
XP_505159.1 | 279/20/92 | 18.3/5.50 | 17.3/5.5 |
| 41 | YALI0C17347p, SSC1 Heat shock protein 647 a.o., fragment |
Q6CBM2 | 148/15/28 | 22.5/6.2 | 70.0/5.4 |
* S/M/C—the Mascot Score is an indicator of conformity or “scorecard”; match peptides are the number of matched peptides; coverage is the percentage of the complete amino acid sequence of the protein using the identified peptides. ** MM/pI (exp.) are the obtained estimates according to electrophoretic mobility in the DE, MM/pI (calc.) are the estimates made using the data on the amino acid sequence, taking into account signal peptide removal, but with no consideration of other post-synthetic modifications using the ExPASy Compute pI/Mw tool software. ‡ Marks the protein in the composition of the mixtures in the protein spots.