Table 4.
The list of down-regulated proteins in response to alkaline stress in the Y. lipolytica yeast using mass spectrometry (MALDI-TOF MS and MS/MS) under alkaline conditions.
| Spot Number | Protein Name | NCBI or UniProt ID | S/M/C * | MM/pI (expt.) ** |
MM/pI (calc.) ** |
|---|---|---|---|---|---|
| 23 | YALI0B03564p, 1,3-beta-glucanosyltransferase activity | Q6CFU7 | 302/23/68 | 33/4 | 34.1/4.5 |
| 29 ‡ | YALI0D2202, ATP synthase subunit delta | Q6C877 | 84/4/73 | 16/4 | 14.8/4.4 |
| 30 ‡ | YALI0E19723p, cytochrome-c oxidase chain IV | Q6C5A3 | 116/10/72 | 15/5 | 17.6/5.2 |
| 31 ‡ | YALI0E10144p, cytochrome-c oxidase subunit VI | Q6C6E6 | 93/15/45 | 12/4.5 | 17/4.8 |
| 32 | YALI0F01496p, Thioredoxin | Q6C399 | 162/16/81 | 10/4.2 | 11.5/4.6 |
| 37 | Ubiquitin-60S ribosomal protein L40 fusion protein, ubiquitin | Q6C2D7 | 91/22/53 | 9/7 | 14.8/9.8 |
* S/M/C—The Mascot Score is an indicator of conformity or “scorecard”; match peptides are the number of matched peptides; coverage is the percentage of the complete amino acid sequence of the protein using the identified peptides. ** MM/pI (exp.) are the obtained estimates according to electrophoretic mobility in the DE, MM/pI (calc.) are the estimates made using the data on the amino acid sequence, taking into account signal peptide removal, but with no consideration of other post-synthetic modifications using the ExPASy Compute pI/Mw tool software. ‡ Marks the protein in the composition of the mixtures in the protein spots.