Table 5.
List of differentially expressed proteins in response to heat stress in the Y. lipolytica yeast using mass spectrometry (MALDI-TOF MS and MS/MS).
| Spot Number | Protein Name | NCBI or UniProt ID | S/M/C * | MM/pI (expt.) ** |
MM/pI (calc.) ** |
|---|---|---|---|---|---|
| 42 | YALI0D22352p, SSA4 heat shock protein | Q6C864 | 112/4/9 | 34/5.0 | 70.1/5.1 |
| 43 | CPAR2_700380, heat shock protein 70 family | G8BL45 | 141/10/27 | 36/5.0 | 61.2/5.0 |
| 43 | CPAR2_106080, Mitochondrial import inner membrane translocase TIM40 | G8B7Y8 | 129/7/18.5 | 36/5.0 | 33/5.2 |
| 44 | YALI0E35046p, SSA4 heat shock protein | Q6C3G5 | 84/5/9 | 23/4.5 | 70.1/5.0 |
| 45 | YALI0F20856p, Cofilin | Q6C0Y0 | 83/2/10 | 18/5 | 17.02/5.3 |
| 46 | CPAR2_203450, Formate dehydrogenase | G8BFR8 | 127/5/15 | 17/5.0 | 15.0/5.7 |
| 47 | CPAR2_401230, Fructose-bisphosphate aldolase | G8BI56 | 137/4/13 | 17/8.0 | 39.8/5.4 |
| 48 | Q0ZIC1_CANPA, GTPase cytoplasmic elongation factor 1 alpha | Q0ZIC1 | 148/6/33 | 23/8.7 | 27.4/8.5 |
| 49 | CPAR2_802980 | G8BC83 | 250/15/27 | 20/4.0 | 56.4/6.5 |
| 50, 51 | YALI0B22506p, Formate dehydrogenase | Q6CDN8 | 86/5/18 | 20/5.3 | 40.3/6.5 |
| 52 | CPAR2_200490, Thioredoxin | G8BEL7 | 172/4/50 | 11/6.5 | 11.3/5.3 |
| 53 | YALI0D22352p, SSA4 heat shock protein | Q6C864 | 173/18/31 | 23/62 | 70.5/5.1 |
| 54 | CPAR2_500390, Cu/Zn SOD | G8BH89 | 201/12/68 | 16/6.5 | 16.2/5.4 |
* S/M/C—the Mascot Score is an indicator of conformity or “scorecard”; match peptides are the number of matched peptides; coverage is the percentage of the complete amino acid sequence of the protein using the identified peptides. ** MM/pI (exp.) are the obtained estimates according to electrophoretic mobility in the DE, MM/pI (calc.) are the estimates made using the data on the amino acid sequence, taking into account signal peptide removal, but with no consideration of other post-synthetic modifications using the ExPASy Compute pI/Mw tool software.