Table 6.
The list of differentially expressed proteins in response to combined stress in the Y. lipolytica cells using mass spectrometry (MALDI-TOF MS and MS/MS).
| Spot Number | Protein Name | NCBI or UniProt ID | S/M/C * | MM/pI (exp.) ** |
MM/pI (calc.) ** |
|---|---|---|---|---|---|
| 55 | CPAR2_503440, ATP synthase subunit 5 | G8BGX1 | 141/3/27 | 20/9.5 | 23.1/9.7 |
| 56 | CPCR1, Carbonyl reductase | H9DXW0 | 92/3/15 | 14/8.7 | 37.1/6.3 |
| 57 | CPAR2_207060, Elongation factor 1-alpha | G8BCR8 | 125/3/7 | 14/8.5 | 50/9.1 |
| 58 | CPCR1, Carbonyl reductase | H9DXW0 | 99/6/16 | 12/8.7 | 37.1/6.3 |
| 59 | CPAR2_101390, Nucleoside diphosphate kinase | G8B6L9 | 142/9/39 | 13/7 | 16.8/8.6 |
| 60 | CPAR2_808670, Glyceraldehyde-3-phosphate dehydrogenase | G8BB94 | 114/7/30 | 13/6.5 | 36.1/6.2 |
| 61 | CPAR2_500390, Cu/Zn SOD | G8BH89 | 148/7/61 | 16/6.9 | 16/5.4 |
| 62 | CPAR2_406210, SBDS domain-containing protein | G8BJK2 | 82/4/30 | 13/5.3 | 12.5/4.8 |
| 63, 64 | CPAR2_700380, heat shock protein 70 family | G8BL45 | 146/13/26 | 30/5 | 61/5.0 |
| 65 | CPAR2_402950, Pyruvate dehydrogenase E1 component subunit beta | G8BIM8 | 142/3/10 | 40/5 | 42.1/5.0 |
| 66 | hsp70 chaperone, heat shock protein 70 family | H8X9S4 | 132/9/38 | 23/5 | 22.7/5.7 |
* S/M/C—the Mascot Score is an indicator of conformity or “scorecard”; match peptides are the number of matched peptides; coverage is the percentahe of the complete amino acid sequence of the protein using the identified peptides.** MM/pI (exp.) are the obtained estimates according to electrophoretic mobility in the DE, MM/pI (calc.) are the estimates made using the data on the amino acid sequence, taking into account signal peptide removal, but with no consideration of other post-synthetic modifications using the ExPASy Compute pI/Mw tool software.