Figure 1.

Expression Analysis of AXL in HCC Cell Lines. (A) The exon representations of the two alternative spliced isoforms of AXL. (B,C) mRNA levels of the long transcript, AXL FL, and short transcript, AXL V, were examined in 10 HCC cell lines using the real-time polymerase chain reaction (qPCR) technique. (D) Total proteins were analyzed in 13 HCC cell lines. 138 kDa AXL protein and shorter AXL protein forms were detected in mesenchymal-like but not epithelial-like HCC cell lines. β-actin was used as a loading control.