Figure 6.

Cytotoxicity effects of LVTX-9 and LVTX-9-C₁₈ on B16-F10 tumor spheroids: (A) Tumor spheroid images on day 0 and day 3. (B) Volume quantitative results of tumor spheroids. (C) Cell viabilities of the tumor spheroids were obtained in the presence of different concentrations of LVTX-9 and LVTX-9-C₁₈ by CellTiter-Glo 3D Cell Viability Assay, respectively (n = 4). Statistical analysis was performed using GraphPad software 5.0 (One-way ANOVA test). ns, non-significant, ** p < 0.01, and *** p < 0.001.