Figure 4.

Protein expression levels of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated BV2 (A) and RAW264.7 (C) cells. The cells were pretreated for 2 h with various concentrations of compounds 3 or 4 and stimulated for 24 h with LPS (1 μg/mL). Representative blots from three independent experiments are shown. Immunoblots were quantified using the ImageJ software. Band intensities are normalized to that of β-actin (B,D). Error bars represent mean ± standard deviation of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the LPS-treated group.