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. 2021 Dec 16;26(24):7629. doi: 10.3390/molecules26247629

Figure 5.

Figure 5

Effect of Carpachromene on the expression of phosphorylated and total proteins of IR, IRS1, IRS2, PI3K, Akt, GSK3, and FoxO1 proteins in HepG2/IRM cells. (A) Representative Western blots of phosphorylated and total proteins of IR, IRS1, IRS2, PI3K, Akt, GSK3, and FoxO1 in HepG2/IRM cells before (left lane) and after (right lane) treatment with 20 µg/mL carpachromene. β-actin was used as internal loading control. (B) Phosphorylated/total protein expression ratio in HepG2/IRM cells relative to untreated HepG2/IRM cells, after normalization to the corresponding β-actin protein expression. Bars represent mean ± SD. Significant difference was analyzed by student t test, where ** p < 0.01, compared to untreated HepG2/IRM cells.