FIG. 6.

Expression of human papillomavirus type 16 E6 protein alleviates Bop1Δ-induced cell cycle arrest. LAP3 cells were cotransfected with pX11-Bop1Δ, the selection marker pPGK-puro, and either pJ4Ω16E6 or pJ4Ω16E6Δ111–115, which drive expression of wild-type E6 and mutant E6 defective in p53 binding, respectively (11), or vector DNA. Pools of stably transfected clones were obtained by puromycin selection, and equal numbers of cells from each pool were subjected to BrdU and light treatment as described in the legend to Fig. 1.