FIGURE 4.
Expression of candidate markers for sorted cell populations. (A) Comparision of germ cell related genes expression profile of P1 cells between stage I and II testes by q-PCR. Gene expressions in stage I were defined as the relative value 1.0. (B) Comparision of germ cell related gene expression profiles of P1 and P2 cells at stage II testes by q-PCR. Gene expressions in P1 were defined as the relative value 1.0. Cq values were normalized per gene for relative quantification using the arithmetic mean of differentiated group values (A) and P1 values (B). . Data are presented as geometric mean value ± geometric standard deviation (GSD). Means with an asterisk indicate a significant difference (n = 3, p < 0.05). (C) Histological observation of early stage testes used for in situ hybridization (ISH). (D) The cellular distributions of plzf by ISH. (E) Merged photograph with DAPI. (F) Histological observation of testes at a later maturing stage used for ISH. (G) The cellular distributions of plzf by ISH. (H) Merged photograph with DAPI.