Figure 3.

(A) Secondary metabolome analyses via HPLC-MS of P. fallax An d48 revealed the target natural product at m/z 222.615 [M + 2H]²⁺, supporting the deduced molecular formula C₂₂H₃₁N₅O₅²⁺ at a retention time of 0.7 min. (B) Production of 1 in different media as observed as an LC-MS EIC at 222.615 ± 0.05 Da [M + 2H]²⁺. The black traces represent media without significant production of 1, namely AMB, YM, CFL, CTT, M and P media. The blue trace represents P medium containing autoclaved Escherichia coli (Pec), the yellow trace represents P medium containing autoclaved Saccharomyces cerevisiae (Py), the green trace represents low-concentration-starch-based medium containing autoclaved S. cerevisiae (VY/2) and red trace represents high-concentration-starch-based medium containing autoclaved S. cerevisiae (VY).