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. 2021 Dec 10;26(24):7483. doi: 10.3390/molecules26247483

Figure 5.

Figure 5

Heterologous production of the myxobacterial biarylitide MeYLH (1) in M. xanthus DK1622. HPLC−MS EIC of crude extracts from P. fallax An d48 wild type as the positive control and M. xanthus DK1622_pFPVan_mx8 mutant strain as the negative control. While the HPLC−MS EIC of the crude extract from M. xanthus DK1622_pFPVan_bytAOZ mutant without vanillate supplementation showed only trace amounts of 1 due to leaky expression, the HPLC−MS EIC of the crude extract from M. xanthus DK1622_pFPVan_bytAOZ without 2 mM vanillate supplementation indicates low but significant production of 1. EIC: extracted ion chromatogram; blue, 222.615 ± 0.05 Da [M + 2H]2+.