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. 2021 Dec 8;22(24):13239. doi: 10.3390/ijms222413239

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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OGT and TET3 dysregulation affect histone modifications. The O-GlcNAcylation of serine 112 of histone H2B (H2BS112GlcNAc), ubiquitination of lysine 120 of histone H2B (H2BK120Ub), and trimethylation of lysine 4 of histone H3 (H3K4me3) have been analyzed using the ChiP method in cells with OGT (A,C,E) or TET3 (B,D,F) overexpression. Differential chromatin enrichment was quantified using Real-time quantitative PCR. The results were calculated as % input (% input = % input of specific antibodies probes—% input mock IgG) and are presented as a log2fold change (Fold change = % input of treated cells sample/% input of control cells sample). Data show mean ± SE, (n = 5) * p < 0.01, ** p < 0.001.