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. 2021 Nov 27;13(12):4281. doi: 10.3390/nu13124281

Figure 5.

Figure 5

Changes in the mRNA expression of porcupine, c-myc, Smox, Bambi, and Smad4 of H. pylori-infected AGS cells with or without ASTX pretreatment. AGS cells were pretreated with vehicle (DMSO) or 5 μM ASTX for 3 h and then incubated with H. pylori at a cellular ratio of 100:1 for 4 h. Real-time PCR assay was performed to measure the relative amounts of porcupine, c-myc, Smox, Bambi, and Smad4 mRNA in AGS cells. mRNA levels were normalized to that of β-actin. * p < 0.05 vs. uninfected cells; + p < 0.05 vs. H. pylori-infected cells without ASTX pretreatment.