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. 2001 Jul;21(13):4292–4301. doi: 10.1128/MCB.21.13.4292-4301.2001

FIG. 1.

FIG. 1

ILP-2 is conserved during the evolution of great apes. (A) Schematic representation of ILP-1 and ILP-2. (B) Sequence comparison of the ILP-1 and ILP-2 proteins. The predicted amino acid sequences of the corresponding region of human ILP-1 (residues 262 to 497) are shown aligned with the full coding sequence of human ILP-2, chimpanzee ILP-2, and gorilla ILP-2. The GenBank accession number of human ILP-2 is AF164682, and the accession numbers of chimpanzee and gorilla ILP-2 are AY030052 and AY030053, respectively. (C) Southern blot analysis of EcoRI-digested genomic DNA from humans, great apes (chimpanzee and gorilla), Old World monkeys (baboon, cynomolgus monkey, and rhesus monkey), and mouse (C57BL6) was obtained by using a probe which detects both ILP-1 and ILP-2. Included in the experiment were the human genomic clones of ILP-1 (PAC–ILP-1) and ILP-2 (BAC–ILP-2). The gorilla ILP-2 gene does not contain an EcoRI site in its coding sequence, accounting for a profile that differs slightly from those of the human and chimpanzee genes.