FIG. 3.

ILP-2 inhibits apoptosis induced by Bax and caspase 9 plus Apaf-1 but not by Fas. Human embryonic kidney 293 cells were transfected with a GFP plasmid (0.5 μg/well) in combination with either a Bax expression plasmid (0.25 μg/well) (A), Fas expression plasmid (2 μg/well) (B), or Apaf-1 (2 μg/well) and caspase 9 (0.5 μg/well) plasmids, as indicated (C). Cells were cotransfected with a plasmid encoding GFP as a marker of transfection, together with a control plasmid or plasmids expressing ILP-1, ILP-2, or dominant-negative caspase 9 (C-9 DN) (2 μg/well) as indicated. Cells were fixed and stained with DAPI, and transfected wells were scored for viability based on nuclear morphology as described in Materials and Methods. Data shown are representative of at least three independent experiments.