Figure 1.

DsRNA is contained within a membrane-bound compartment whilst nsp12 is exposed to the cytoplasm. DF1 cells were infected with IBV and fixed at the indicated times post infection. Cells were permeabilized with either Triton X-100 (all membranes permeabilized; top row), or digitonin (plasma membrane permeabilized only; bottom row). Cells were labeled with dsRNA (red) and nsp12 (green), or for the mock control (first column), tubulin (red) and PDI (green). Nuclei are labeled blue with DAPI (blue). Scale bars represent 5 µm. Images are representative of 3 independent replicates.