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. 2021 Dec 17;13(12):2540. doi: 10.3390/v13122540

Figure 2.

Figure 2

Sites of viral RNA synthesis are associated with dsRNA, but do not colocalize with nsp12. DF1 cells were infected with IBV and 30 min prior to fixation treated with BrU and ActD. Cells were fixed at the indicated time points post infection and labeled with antibodies against BrU (green) and (a) nsp12 (red) or (b) dsRNA (red). Nuclei are labeled with DAPI (blue). Scale bars represent 5 µm. Images are representative of three independent replicates; (c,d) cells were treated as in (a,b), fixed at 10 hpi and labeled for BrU (green) and (c) nsp12 or (d) dsRNA (red) and ToPro3 (blue) for the nuclei. A confocal image was captured followed by a super-resolution image, which was captured using a STED microscope, then deconvolved. Scale bars represent 3 µm.