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Physical detection of RAG target capture complexes. (A) Schematic of assay employed to detect noncovalently associated RAG-donor-target complexes. The asterisk indicates the position of radiolabel. (B) Lanes marked + RAG contain RAG proteins, HMG1, 12- and 23-donor RSS, target, and Mg2+. TCC denotes the position of target capture complexes. All reaction mixturess were loaded directly to gel without extraction. ProtK, proteinase K; wt, wild type.
