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. 2021 Nov 30;13(12):4330. doi: 10.3390/nu13124330

Figure 2.

Figure 2

Hemp seed extract increases β-catenin and p-ERK1/2 pathways protein levels on Saos-2 cells. (a) Semi-confluent cultures of human osteoblast-like cells (Saos-2) incubated with HSE 5, 10, or 20 µg/mL for 24 hours. Cell proteins were analyzed by Western blotting with antibodies specific to β-catenin and β-actin. (b) Semi-confluent cultures of human osteoblast-like cells (Saos-2) were incubated with HSE 5, 10, or 20 µg/mL for 10 min. Cell proteins were analyzed by Western blotting with antibodies specific to phosphorylated ERK1/2 and β-actin. Data are represented as mean ± SD. Statistical analysis: Student’s t-test vs. 0 * p < 0.05, Linear regression # p < 0.05.