Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 30;13(12):4330. doi: 10.3390/nu13124330

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

HSE downregulates RANKL and upregulates RUNX2 and Osteocalcin mRna expression levels on Saos-2 cells. Semi-confluent cultures of human osteoblast-like cells (Saos-2) were incubated with HSE 5, 10, or 20 µg/mL for 24 h. The mRNA expression levels of RANKL (c), OPG (d), RUNX2 (a), and Osteocalcin (b) were measured by RT-PCR. Data were analyzed using the 2-ΔΔCq method and normalized to β-actin. Data are represented as mean ± SD. Statistical analysis: Student’s t-test vs. 0 * p < 0.05; ** p < 0.01; *** p < 0.001, Linear regression # p < 0.05.