Skip to main content
. 2021 Dec 16;22(24):13515. doi: 10.3390/ijms222413515

Figure 1.

Figure 1

Cumulative population doublings of all paired DPSCs reached from the primary to the 8th passage. We used the formula PD = log2 (Nx/N1) for calculating the population doublings reached in each passage. Nx is the total passage cell count calculated using the Z2-Counter (Beckman Coulter, Miami, FL, USA), and N1 is the initial cell count seeded into the culture dish (5000 cells/cm2). The statistical significance was calculated using a paired t-test; no comparison was statistically significant.