Figure 1.

Schematic diagram of the parallel metabolomic and lipidomic workflows used in this study. Serum samples were collected from patients, and aliquoted for separate extraction of metabolites and lipids. The extracted samples were analyzed by LC-MS/MS using C18 and HILIC columns (metabolomics) and C30 column (lipidomics). Raw MS and MS/MS data files were acquired by high resolution mass spectrometer on Orbitrap QE-HF and processed by Compound Discoverer (metabolomics) and LipidSearch (lipidomics). Further statistical and bioinformatics analyses were performed to identify altered metabolites/lipids and potential mechanistic pathways in response to benfotiamine treatment.