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. 2021 Dec 7;13(12):873. doi: 10.3390/toxins13120873

Figure 1.

Figure 1

Upregulation of lnc-SLC15A1-1 in the high-glucose-cultured HUVECs after indoxyl sulfate treatment. (A) MTT assay. Proliferation of HUVECs was examined in 30 mM D-glucose (HG) or HG with 0.1 mM indoxyl sulfate (HG + IS) for 4 days. (B) Principal component analysis (PCA) of samples. RNA sequencing was performed in HG + IS and HG groups (n = 3). Log-transformed fragments per kilobase of transcript per million mapped reads were used to perform PCA. (C) Volcano plot of differentially expressed lncRNAs. Selection criteria: fold change ≥1.5 or ≤−1.5 with p-value < 0.05. (D) Heatmap and hierarchical cluster analysis of 208 differentially expressed lncRNAs. Each column represents one sample, and each row represents one lncRNA. Red: upregulated lncRNAs; green: downregulated lncRNAs. (E) Relative expression levels of lnc-SLC15A1-1 by quantitative RT-PCR. (* p < 0.05, ** p < 0.01).