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. 2021 Dec 7;13(12):873. doi: 10.3390/toxins13120873

Figure 3.

Figure 3

Identification of genes regulated by lnc-SLC15A1-1 in HUVECs by microarray analysis. (A) Relative expression levels of lnc-SLC15A1-1 by quantitative RT-PCR. HUVECs were transfected with 1.5 μg of lnc-SLC15A1-1 expression plasmids (pcDNA3.1(+)-lnc-SLC15A1-1). The lnc-SLC15A1-1 RNA expression levels were normalized to 18S rRNA (*** p < 0.001). (B) Principal component analysis. HUVECs were transfected with empty vector (EV; red spots) or lnc-SLC15A1-1 expression plasmids (Lnc; blue spots). The expression levels were analyzed by Affymetrix Clariom™ S Assay (n = 3). The expression levels of total mRNAs were used for plotting PCA. (C) Volcano plot of differentially expressed genes (DEGs) in HUVECs over-expressing lnc-SLC15A1-1. DEGs with fold change ≥1.5 or ≤−1.5 and p-value < 0.05 are labeled as red or green spots, respectively. (D) Heatmap with hierarchical clustering. Red color indicates upregulated genes; green color indicates downregulated genes.