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. 2021 Dec 7;13(12):873. doi: 10.3390/toxins13120873

Figure 7.

Figure 7

Lnc-SLC15A1-1 functions as a miRNA sponge. (A) Venn diagram of miRNAs predicted to interact with lnc-SLC15A1-1, CXCL10, and CXCL8. MiRNAs were predicted by miRDB (http://mirdb.org/index.html, accessed on 17 December 2020). (B) Relative expression levels of three predicted miRNAs. The expression levels of miRNAs in HUVECs overexpressing lnc-SLC15A1-1 or empty vector (pcDNA3.1(+)) were detected by quantitative RT-PCR and normalized to U6. Data are the means ± SDs (* p < 0.05, ** p < 0.01, and *** p < 0.001). (C) RNA immunoprecipitation (RIP) analysis of lnc-SLC15A1-1 using antibody against Argonaute-2 (AGO2) in HUVECs. The RIP enrichment of the AGO2-associated lncRNA was measured by quantitative RT-PCR and normalized to U6. The relative fold enrichment was calculated as compared to the IgG group. Data are the means ± SDs (n = 5 and ** p < 0.01, *** p < 0.001).