FIG. 5.

Mislocalization of Faa2p to the cytosol complements the MCFA β-oxidation defect in ypr128cΔ cells. Cells were grown on oleate-containing medium and incubated with 1-¹⁴C-labeled laurate, and β-oxidation activity was measured (see Materials and Methods). The β-oxidation rates in wild-type (WT) cells were taken as the reference (100%) and are expressed as the sum of [1-¹⁴C]CO₂ and water-soluble β-oxidation products. Each experiment was performed at least two times, and the means are shown by error bars.