Table 2.
Combination of independent variables in THQ–PL–SV phytosomes experimental runs, prepared according to 32 factorial design, and their corresponding responses.
| Run | X1 | X2 | Y1 | Y2 |
|---|---|---|---|---|
| PL Concentration | SV Concentration | Vesicle Size * ± SD | Zeta Potential * ± SD | |
| (mg) | (mg) | (nm) | (mV) | |
| 1 | 79.0 | 170.0 | 198.8 ± 9.8 | 26.8 ± 7.4 |
| 2 | 395.0 | 34.0 | 388.0 ± 13.6 | 2.7 ± 4.1 |
| 3 | 395.0 | 102.0 | 412.4 ± 24.1 | 17.7 ± 8.7 |
| 4 | 395.0 | 170.0 | 435.6 ± 19.3 | 11.8 ± 4.6 |
| 5 | 79.0 | 34.0 | 143.7 ± 14.6 | 4.4 ± 3.9 |
| 6 | 79.0 | 102.0 | 176.6 ± 17.9 | 6.7 ± 3.7 |
| 7 | 237.0 | 34.0 | 212.5 ± 13.5 | 3.8 ± 4.7 |
| 8 | 237.0 | 170.0 | 298.8 ± 23.3 | 9.8 ± 5.2 |
| 9 | 237.0 | 102.0 | 274.5 ± 19.9 | 7.9 ± 6.7 |
| 10 | 79.0 | 170.0 | 199.7 ± 21.1 | 27.1 ± 5.4 |
| 11 | 395.0 | 34.0 | 389.7 ± 26.4 | 2.9 ± 7.3 |
| 12 | 395.0 | 102.0 | 410.3 ± 28.7 | 18.9 ± 5.7 |
| 13 | 395.0 | 170.0 | 430.5 ± 23.1 | 11.8 ± 3.4 |
| 14 | 79.0 | 34.0 | 145.8 ± 12.5 | 4.1 ± 5.3 |
| 15 | 79.0 | 102.0 | 174.6 ± 23.8 | 6.6 ± 4.3 |
| 16 | 237.0 | 34.0 | 214.7 ± 18.9 | 3.8 ± 4.7 |
| 17 | 237.0 | 170.0 | 298.4 ± 22.2 | 9.9 ± 5.1 |
| 18 | 237.0 | 102.0 | 276.8 ± 24.3 | 7.8 ± 3.6 |
Abbreviations: THQ, thymoquinone; PL, Phospholipon® 90H; SV, scorpion venom peptide; SD, standard deviation. * Results are presented as mean ± SD, n = 3.