Figure 5.
Effects of EEB on ROS production and the antioxidant enzymes expression through Nrf2 signaling in UVB-induced Hs68 cells. Cells were irradiated with UVB and then treated with various concentrations of EEB (25, 50, and 100 µg/mL). (A, B) Productions of intracellular ROS were stained with H2DCFDA and analyzed by flow cytometry. (C) Whole-cell lysates and cytosolic and nuclear fractions were analyzed by Western blotting to determine the levels. Protein expression of GPx1, HO-1, and Nrf2. Protein levels of GPx1, HO-1, and cytosolic fraction Nrf2 normalized to β-actin and nucleus fraction Nrf2 normalized to Histone H3 were determined using Bio-Rad Quantity One software (Basic; Bio-Rad Laboratories Inc., Hercules, CA, USA). Values are represented as mean ± SD. # p < 0.05 vs. the non-UVB-irradiated control group; *** p < 0.001 vs. the UVB-irradiated group.