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Flavonoids decreased the inflammatory levels caused by Hla in vitro detection: Pulmonary microvascular endothelial cells (PMVECs) were treated Hla with 4 h (A) ASC, Caspase-1 and NLRP3 expressions of Kaempferol and quercetin treatment were detected by Western Blot. (B) The corresponding proteins analyzed from A were normalized to the of levels of β-actin. (C) The IL-1β and IL-18 levels were determined by ELISA assay.
