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. 2021 Dec 20;10(12):2815. doi: 10.3390/plants10122815

Figure 1.

Figure 1

Self-compatibility phenotype identification and S haplotype determination of the inbred line ‘325’. (A) self-pollination of inbred line ‘325’ by observing with aniline blue staining, scale bar = 100 μm; (B) self-pollination of inbred line ‘325’ by observing silique development, scale bar = 2 cm; (C) S haplotype identification with class I and class II universal primer pairs, PS5 and PS15 and PK1 and PK4 were used for class I S haplotypes identification, PS3 and PS21 and SRK IIE4L and E7R were used for class II S haplotypes identification, with Actin7 as the control. (D,E) phylogenetic analysis of class II S locus genes SCR and SRK E1. An unrooted phylogenetic tree was constructed using the neighbor-joining method. Bootstrap values from 1000 replicates are shown.