Table 1. Results from the milestones protocol tests included in the assessment of early postnatal neurodevelopment.
Sample size: nWT = 9; n AP2γ KO = 9. Abbreviations: WT, wild-type; AP2γ KO, AP2γ heterozygous mice; P, Postnatal day.
| Milestone test | WTDay (median) | AP2γ KODay (median) | Statistical test, significanceMann-Whitney test | Typical range | |
|---|---|---|---|---|---|
| Rooting | 7 | 7 | U = 31.50, p = 0.43 | P1 – P15 | |
| Ear twitch | 8 | 9 | U = 24.50, p = 0.16 | P6 – P14 | |
| Auditory startle | 8 | 7 | U = 37.50, p = 0.85 | P7 – P16 | |
| Open field | 8 | 8 | U = 39.50, p > 0.99 | P6 – P15 | |
| Walking | 10 | 9 | U = 38.00, p = 0.78 | P7 – P14 | |
| Surface righting | 7 | 7 | U = 38.50, p = 0.88 | P1 – P10 | |
| Negative geotaxis | 8 | 9 | U = 25.00, p = 0.17 | P3 – P15 | |
| Cliff aversion | 7 | 6 | U = 26.00; p = 0.22 | P1 – P14 | |
| Postural reflex | 9 | 8 | U = 26.00, p = 0.21 | P5 – P21 | |
| Air righting | 12 | 11 | U = 29.00, p = 0.32 | P7 – P16 | |
| Wire suspension | 13 | 11 | U = 21.50, p = 0.10 | P5 – P21 | |
| Grasping | 16 | 16 | U = 30.00, p = 0.37 | P13 - P17 | |
| Eye-opening | 12 | 11 | U = 9.00, p < 0.01 | P7 - P17 | |
|
WT Mean ±SEM |
AP2γ KO Mean ± SEM |
Statistical test, significance Repeated measures ANOVA |
|||
| Homing | Trial 1 | 6.89 s ± 0.44 | 6.89 s ± 1.32 | F (1,48) = 0.06, p = 0.80 | |
| Trial 2 | 6.33 s ± 1.12 | 6.89 s ± 1.12 | |||
| Trial 3 | 5.22 s ± 1.57 | 5.78 s ± 1.57 | |||
| Weight gain pattern | 8.99 g ± 0.53 | 9.22 g ± 0.16 | F (1,16) = 0.32, p = 0.58 | ||