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. 2021 Dec 24;10:e72747. doi: 10.7554/eLife.72747

Figure 1. Sampled locations for Hyalomma marginatum ticks.

Sampling countries are colored in gray. The symbiont genomes retrieved from each site are graphically represented in blue, orange, and pink, corresponding to Francisella-LE (FLE), Midichloria (Midi), and R. aeschlimannii (RAES), respectively. Circles on genome maps correspond to the following: (1) forward strand genes; (2) reverse strand genes; (3) in gray and black, contigs. Circular maps of the genomes were produced using CGView.

Figure 1.

Figure 1—figure supplement 1. Rickettsia phylogenetic tree assessing the affiliation of the Rickettsia detected in Hyalomma marginatum specimens from Italy and Israel.

Figure 1—figure supplement 1.

The phylogenetic tree was inferred using maximum likelihood from a concatenated alignment of 324 single-copy orthologs genes (88,128 amino acids; best-fit approximation for the evolutionary model: JTT+G4+I). The numbers on each node represent the support of 1000 bootstrap replicates; only bootstrap values >70% are shown. The scale bar is in units of substitution/site. Sequence from the newly sequenced Rickettsia genome is indicated by a pink font.
Figure 1—figure supplement 2. Phylogenetic relationship of the Hyalomma species used in our study.

Figure 1—figure supplement 2.

The phylogenetic tree was inferred using maximum likelihood from an alignment of the cytochrome c oxidase I (cox1) gene sequences (546 bp unambiguously aligned, best-fit approximation for the evolutionary model: HKY+G+I). The numbers on each node represent the support of 1000 bootstrap replicates; only bootstrap values >80% are shown. The scale bar is in units of substitution/site.
Figure 1—figure supplement 3. Presence of FLE and Midichloria within the Hyalomma genus.

Figure 1—figure supplement 3.

The Hyalomma cladogram was adapted from cox1 phylogeny showed in Figure 6. The numbers on each node represent the support of 1000 bootstrap replicates; only bootstrap values >80% are shown. The scale bar is in units of substitution/site. Blue and orange plain circles indicate the presence of FLE and Midichloria, respectively, as detailed in Supplementary file 1d. Blank circle represents an absence of symbiont.