FIG. 7.

Functional analysis of SRC1e VHC ΔAD1. (A) Ligand-dependent interaction of mERα V380H with SRC1e VHC ΔAD1 in vitro. Coimmunoprecipitation was carried out as described for Fig. 2D. The input control represents 2% of the whole-cell extract employed in the immunoprecipitation (IP) reaction. wt, wild type. (B) Wild-type and mutant chimeric receptors consisting of the mERα LBD fused to the Gal4 DBD were transiently transfected into HeLa cells together with the p5Gal-E1B-GL3 reporter in the absence (−) or presence of full-length SRC1e VHC or SRC1e VHC ΔAD1 as indicated. The pRL-CMV plasmid was cotransfected as an internal control. Data are presented as described for Fig. 3A. NH, no hormone.