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. Author manuscript; available in PMC: 2021 Dec 24.
Published in final edited form as: Cell Rep. 2021 Sep 28;36(13):109758. doi: 10.1016/j.celrep.2021.109758

Figure 2. scRNA-seq analysis of the response of the SGNs to PTS-inducing noise exposure.

Figure 2.

(A) UMAP of the 8,916 SGNs and Schwann cells (n = 4 biological replicates for each condition).

(B) Violin plots for the expression of known marker genes, colored according to cell type as in (A).

(C and D) Upset plots of upregulated (C) and downregulated (D) DEGs in noise-exposed versus control samples. Horizontal bars: overall number of DEGs detected in each cell type. Vertical bars: number of DEGs in selected intersections between cell types indicated below the bars.

(E) Hierarchical clustering applied to the set of 56 upregulated genes that showed, upon noise exposure, a fold-change induction greater than 1.5 and FDR q value <0.05 (MAST’s statistical test) in at least one of the cell types. The grid above the heatmap displays assignment of genes to enriched GO terms (q < 0.05, hypergeometric test) (Table S3).

(F) Same as (E) but for the 27 downregulated genes (Table S3).

(G) The GO term “synapse” is enriched in the downregulated DEGs of type 1A. (q < 0.05, hypergeometric test).