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. 2021 Dec 17;2021:6370911. doi: 10.1155/2021/6370911

Figure 4.

Figure 4

IL-38 inhibits LPS-induced apoptosis of macrophages. (a) Representative flow cytometry plots of macrophages treated with PBS or LPS (1 μg/mL; 24 h) alone or with recombinant IL-38 (200 ng/mL; 24 h) in order to evaluate apoptosis (left panel). Percentages of apoptotic (Annexin V+) macrophages (right panel). (b) Representative western blot image (left panels) and quantification (right panels) of the indicated proteins in macrophages stimulated with LPS (1 μg/mL; 24 h) and/or IL-38 (200 ng/ml; 24 h). β-Actin protein was used for normalization. Normal control (NC) group was PBS treatment. ∗P < 0.05 and ∗∗P < 0.01, vs. the NC group; #P < 0 05 and ##P < 0.01, vs. the LPS group. n = 3 independent experiments.