Table 2.

Prediction of the mutational effects on the structural stability. A negative value indicates destabilizing effect.

Index	Protein	Amino Acid Changes	SVM2 Prediction Effect	DDG Value (kcal/mol)
1	Surface Glycoprotein	N74K	Decrease	−0.49
2	Surface Glycoprotein	D614G	Decrease	−0.93
3	Surface Glycoprotein	V622F	Decrease	−1.01
4	Surface Glycoprotein	Q677H	Decrease	−0.67
5	Surface Glycoprotein	S943T	Decrease	−0.24
6	Surface Glycoprotein	D1153	Decrease	−1.08
7	Nucleocapsid phosphoprotein	P13L	Decrease	−0.48
8	Nucleocapsid phosphoprotein	R203K	Decrease	−0.93
9	Nucleocapsid phosphoprotein	G204R	Decrease	−0.52
10	Nucleocapsid phosphoprotein	T205I	Decrease	−0.53
11	ORF1AB Polyprotein	R207C	Decrease	−0.60
12	ORF1AB Polyprotein	K292E	Decrease	−0.42
13	ORF1AB Polyprotein	V378I	Decrease	−0.23
14	ORF1AB Polyprotein	S944L	Decrease	0.63
15	ORF1AB Polyprotein	T1246I	Increase	0.14
16	ORF1AB Polyprotein	K1305 N	Increase	−0.10
17	ORF1AB Polyprotein	Q2702H	Decrease	−0.68
18	ORF1AB Polyprotein	G3278S	Decrease	−0.89
19	ORF1AB Polyprotein	L3606F	Decrease	−1.00
20	ORF1AB Polyprotein	A4489L	Decrease	−0.67
21	ORF1AB Polyprotein	P4715L	Decrease	−0.83
22	ORF1A Polyprotein	R207C	Decrease	−0.60
23	ORF1A Polyprotein	K292E	Decrease	−0.42
24	ORF1A Polyprotein	S318I	Increase	0.36
25	ORF1A Polyprotein	D448 N	Decrease	−0.98
26	ORF1A Polyprotein	V627F	Decrease	−1.19
27	ORF1A Polyprotein	S944L	Increase	0.63
28	ORF1A Polyprotein	T1246I	Increase	0.14
29	ORF1A Polyprotein	K1305 N	Increase	−0.10
30	ORF1A Polyprotein	P2965L	Decrease	−0.67
31	ORF1A Polyprotein	G3278S	Decrease	−0.89
32	ORF1A Polyprotein	L3606F	Decrease	−1.00
33	0RF3A Polyprotein	Q57H	Decrease	−0.90