Fig. 3.

[¹¹¹In]In-DOTA-JR11 uptake in human carotid endarterectomy (CEA) tissue after incubation with [¹¹¹In]In-DOTA-JR11. A Transverse SPECT/CT image of a 2 mm slice of a CEA sample. Calcifications are visible in CT in white, the asterisk indicates the holder used to keep the 2 mm slice in place. B, C In vitro autoradiography of adjacent 10 µm sections made of an adjacent 2 mm slice of the same CEA sample seen in (A). The section in B was incubated with 10⁻⁹ M [¹¹¹In]In-DOTA-JR11, the section in C was incubated with 10⁻⁹ M [¹¹¹In]In-DOTA-JR11 plus a blocking dose of 10⁻⁶ M unlabeled DOTA-JR11. The inset shows the boxed region at higher magnification, the red arrows indicate sectioning artifacts (tissue folds). D SST₂ and E CD68 immunohistochemistry on 5 µm sections adjacent to B and C, with matching insets. Scale bar indicates 2 mm