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. 2021 Dec 24;11:24414. doi: 10.1038/s41598-021-03618-9

Figure 3.

Figure 3

SOCS1 deletion lowers the detection limit of clone H-17 cells. (A) Graphs depicting CRISPR/Cas-9-mediated SOCS1−/− and targeting strategy for SOCS1 exon 1. (B) Western blots showing that SOCS1 was absent in H-17 SOCS1−/− cells compare to H-17 cells [upper panel]. See also Supplementary Fig. S3B for uncropped, full-size original Western Blot. Representative flow cytometry histograms for TLR2 and TLR4 on H-17, H-17 Mock and H-17 SOCS1−/− cells. Percentage of positive cells is indicated on corresponding panels. Histograms are normalised to the mode [lower panel]. (C). Quantification of mCFP expression in H-17 SOCS1−/− clone 3. Bar graphs represent MFI ± SEM. (D-E) Quantification of H-17 SOCS1−/− [brown] response to different concentrations of Pam3CSK4 (D) or FSL-1 (E) compared to H-17 [blue] and H-17 Control [gray] respectively. Bar graphs represent MFI ± SEM. (F). Quantification of mCFP expression kinetic in H-17 SOCS1−/− [green line] and H-17 cells [blue line] upon stimulation with Pam3CSK4 (100 ng/ml) [filled circles] and FSL-1 (10 nM) [open squares] for 0–12 h. Line curves represent MFI.