Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 24;11:24417. doi: 10.1038/s41598-021-03963-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Flow cytometry analysis of candidate mesenchymal markers identifies distinct CD105⁺CD90⁺ populations based on CD13 expression. (A) Schematic of experimental design for analysis of surface marker candidates in primary CD105⁺CD90⁺ mesenchymal cells from human lung parenchyma. (B) Multicolor flow cytometry analysis showing gating of CD105⁺CD90⁺ mesenchymal cells in freshly isolated cells. The plot is shown after forward-scatter/side-scatter gating, exclusion of dead cells (7AAD), hematopoietic cells (CD45) and endothelial cells (CD31), and doublet exclusion (area versus height in forward-scatter). (C) Plots show candidate marker expression (x-axis) versus side-scatter (y-axis) in CD90⁺CD105⁺ mesenchymal cells. For markers CD10, CD13, and CD26, gates are set to define separated population based on marker expression profile. For markers CD44, CD166 and CSPG4, gates are set based on fluorescence minus one (FMO) controls. (D) Plots demonstrate gating for identification of CD105⁺CD90⁺CD13⁻ and CD105⁺CD90⁺CD13⁺ cells in cryopreserved primary/native cells from human lung parenchyma. The plots are shown after forward-scatter/side-scatter gating, exclusion of dead cells (7AAD), red blood cells (CD235a), and hematopoietic cells (CD45). Percentage of cells in each gate out of all cells in the plot, is indicated next to the gates. (E) Frequency of CD13⁻ and CD13⁺ cells in CD105⁺CD90⁺ mesenchymal cell population from healthy parenchymal lung tissue (n = 4). Bars represent mean (with SD). Paired two-tailed t-test was used for statistical analysis of data (*p < 0.05). SSC-A side scatter-area.