Fig. 6.

Cordycepin and cisplatin synergistically inhibit the growth of osteosarcoma xenografts in vivo. a–c Examination of tumor volumes and weight to evaluate the effect of different treatments (Vehicle, cordycepin 40 mg/kg, cisplatin 5 mg/kg, or the combined treatment with both cordycepin 40 mg/kg and cisplatin 5 mg/kg) on 143B cells in a xenograft model. The tumor volume of all groups was measured every 3 days. The tumor and the statistical analysis of tumor volumes in different groups was shown. Data represent the mean ± SD of tumor volume and weight of six mice; d Immunohistochemistry staining of Ki-67 in mouse tumor samples to examine the inhibitory effect on osteosarcoma cell proliferation by cordycepin or/and cisplatin treatment. Combined treatment of cordycepin and cisplatin had a significant decrease in the expression of Ki-67 in vivo. The data are expressed as the mean±SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, by Student’s t-test, scale bars=100 μm