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Immunological evaluation in the long-term immunization. Blood samples were drawn during the experiment from retro-orbital veins and PBMCs were isolated. Interferon-γ (IFN-γ) secreting T cells were evaluated in vitro after O/N re-stimulation with individual peptides. Pool of samples were used for each experimental groups. A–C B16 group; D–F TC1 group. WT NEG animals in the WT group with no sign of tumor growth, HET NEG animals in the Heteroclitic group with no sign of tumor growth
