FIG. 2.

CFP-LacER recruits YFP–SRC-1. (A) A03_1 cells were cotransfected with CFP-LacER (top row) and YFP–SRC-1 (bottom row) and subjected to live microscopy during hormone addition. Before hormone addition (left column), CFP-LacER is found associated with the array, but much of the YFP–SRC-1 remains nucleoplasmic. Addition of E2 (10 nM, 5 min) results in the rapid recruitment of YFP–SRC-1 to CFP-LacER (bottom). (B) When the same experiment is performed with RRE_B1 cells, much less YFP–SRC-1 (bottom left) is evident on an extended, euchromatic array containing CFP-LacER (top left) in the absence of hormone. Addition of E2 (10 nM, 5 min) results in the rapid recruitment of YFP–SRC-1 (bottom right) to the CFP-LacER (top right). Images are deconvolved and represent a single Z-section. Bar = 10 μm.