FIG. 3.

YFP-SRC780 recruitment to CFP-LacER in A03_1 cells. (A) In the absence of hormone, YFP-SRC780 (right column) is present in the nucleoplasm and also accumulates on the CFP-lacER (left column) bound to the lac operator arrays. (B) Following E2 addition (10 nM, 30 min), most of the YFP-SRC780 is found associated with CFP-LacER. (C) Pretreatment with 4HT (10 nM, 30 min) leads to less YFP-SRC780 on the array. (D) Pretreatment with ICI 182,780 (10 nM, 30 min) also diminishes the amount of YFP-SRC780 on the array. (E) Mutation of the LXXLL motifs to LXXAA results in no SRC-1 accumulation on the array even in the presence of agonist. Bar = 10 μm.