Fig. 2.

EXi-RVFV Contain Viral RNA and Select Viral Proteins. A Total RNA extracts from the EXu and EXi-RVFV samples were subjected to RT-qPCR analysis using RVFV-specific primer pairs. Absolute quantitation of the samples was performed using the cycle threshold (Ct) value relative to the standard curve. Mean values ± SEM from three biological replicates are shown. B The results of mass spectrometry analysis of EXi-RVFV to identify associated viral proteins are shown. EXu samples were included as negative control. Mass spectral counts for each identified viral protein is reported. For each sample (EXu or EXi-RVFV), two biological replicates were analyzed. C EXi-RVFV samples were analyzed by western blot for the presence of the viral L, NSs, N, and G proteins. WLC corresponds to whole cell lysate preparations from RVFV-infected Vero cells that were included as positive control. Two biological repeat samples (1 and 2) were analyzed