FIG. 2.

Mapping of the Pex19p-binding sites of Pex3p, Pex12p, Pex13p, and Pex16p. Deletion constructs of Pex3p, Pex12p, Pex13p, and Pex16p fused to Gal4p-BD were tested for interaction with Pex19p fused to the Gal4p-AD in the yeast two-hybrid system. Double transformants were selected and assayed for β-galactosidase activity by using a filter assay with X-Gal as the substrate. The colony staining times were less than 2 h (+++), less than 5 h (++), or less than 10 h (+) or the colonies did not stain at all (−). TMDs are shaded. The smallest delineated Pex19p-BDs are hatched with vertical lines. Residue numbers are on top and on the left. X, alterations of the Pex12p C₃HC₄ RING residues from C-1 to W at position 304 and C-2 to Q at position 307.