FIG. 9.

Identification of the peroxin-binding sites on Pex19p. Deletion mutants of Pex19p fused to Gal4p-AD were tested for interaction with Pex3p, Pex10p, Pex11pβ, Pex12p, Pex13p, and Pex16p fused to Gal4p-BD in the yeast two-hybrid system. Double transformants were selected and assayed for β-galactosidase activity by using a filter assay with X-Gal as the substrate. The colony staining time was less than 2 h (+++), less than 5 h (++), or less than 10 h (+) or the colonies did not stain at all (−). The farnesylation consensus sequence CaaX is shaded in black.